Role of mitogen activated protein kinase cascade pathway and anti-stress response in liver transplantation

摘要

OBJECTIVE: To explore the changes of mitogen activated protein kinase (MAPK) cascade pathway andanti-stress response of hepatocytes after liver transplantation.METHODS: Ten normal liver specimens and 18 punctured donor liver specimens were divided into 3groups: A (control: 10), B (no rejection: 10) and C (acute rejection:8). MAPK, Ras, Jun and heatshock protein 70 (HSP70) were tested immunohistochemically while Ras and HSP70 were tested by in situhybridization. All sections were subjected to image analysis.RESULTS: Protein expressions of MAPK, Ras and Jun were increased by an ascending order of groupsA, B and C. The protein expression of HSP70 was the highest in group B but lower in group C.Expressions of Ras and HSP7O mRNA were consistent with those of protein.CONCLUSIONS: The changes of the MAPK cascade pathway and anti-stress response of hepatocytesafter liver transplantation are one of regulation mechanisms for protecting the hepatocytes from damageafter liver transplantation. This mechanism is active to support individual survival.