Objective: To find out an optimal condition for isola-tion and primary culture of hepatocytes.Method: Rat hepatocytes were isolated by a two-stepcollagenase perfusion, and cultured in hepatozyme-SFM. The reduction of MTT to formazan salt was ex-amined. Supernatant medium was collected for analy-sis of alanine aminotransferase(ALT) and ureagene-sis.Results: The two-step collagenase perfusion yielded39±12×10~6 cells/g liver tissue with a viability of88%±2%. Fine morphology and stable urea synthesisfor one week could be achieved when hepatocytes werecultured in Hepatozyme-SFM.Conclusion: High yield of hepatocytes can be isolatedwith two-step collagenese perfusion. Hepatozyme-SFM is suitable for sustained growth of hepatocytes.
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机译:营造安全文化 提升安全理念 精心打造三峡工程安全文化品牌Creating a Safety Culture,Promoting a Safety Idea and Elaborately Establishing a Safety Culture Brand for the Three Gorges Project
机译:Examining Culture in International Business Curricula: Teaching East Asian Culture Within Universities in the United States =国际商务课程中的文化考察:在美国大学内传授东亚文化