Hepatic ischemic preconditioning increases portal vein lfow in experimental liver ischemia reperfusion injury

摘要

BACKGROUND: Ischemic preconditioning (IPC) has been shown to decrease liver injury and to increase hepatic microvascular perfusion after liver ischemia reperfusion. This study aimed to evaluate the effects of IPC on hemodynamics of the portal venous system. METHODS: Thirty-two  rats  were  randomized  into  two groups: IPC group and control group. The rats of the IPC group underwent IPC by 10 minutes of liver ischemia followed by 10 minutes of reperfusion before liver ischemia, and the rats of the control group were subjected to 60 minutes of partial liver ischemia. Non-ischemic lobes were resected immediately after reperfusion. The animals were studied at 4 hours and 12 hours after reperfusion. Mean arterial pressure, heart rate, portal vein lfow and pressure were analyzed. Blood was collected for the determination of the levels of aspartate aminotransferase, alanine aminotransferase, calcium, lactate, pH, bicarbonate, and base excess. RESULTS: IPC increased the mean portal vein lfow at 4 hours and 12 hours after reperfusion. IPC recovered 78% of the mean portal vein lfow at 12 hours after reperfusion. IPC decreased the levels of aspartate aminotransferase, alanine aminotransferase and  lactate,  and  increased  the  levels  of  ionized  calcium, bicarbonate and base excess at 12 hours after reperfusion. CONCLUSIONS: This study demonstrated that IPC increases portal  vein  lfow  and  enhances  hepatoprotective  effects  in liver  ischemia  reperfusion.  The  better  recovery  of  portal vein lfow after IPC may be correlated with the lower levels of transaminases and with the better metabolic proifle.