目的:探讨维生素A对角膜移植排斥反应后结膜杯状细胞的影响.方法:建立大鼠角膜移植排斥反应模型,随机分3组:A,B,C组为SD-wistar大鼠行同种异体角膜移植术,SD鼠为受体,wistar鼠为供体,其中A组为空白对照组,B组为(维生素A)诺沛凝胶滴眼液组,C组为1g/L地塞米松滴眼液组,另设D组为正常眼组.术后不同时间裂隙灯显微镜记录及比较各组角膜移植排斥指数(Rejection Index,RI).通过结膜组织学切片HE,PAS染色,并运用显微图像分析系统对结膜上皮杯状细胞数量及形态进行分析.结果:观察期间检测结膜杯状细胞发现,A,B,C组杯状细胞数量均少于D组(P＜0.01);A,B,C组内部比较,以C组数量为多,其次为B组,有显著性差异(P＜0.05).结论:维生素A具有保护角膜移植引起的结膜杯状细胞减少的作用.%·AIM: To investigate the effect of vitamin A on the conjunctival goblet cells of rat after corneal transplantation.·METHODS: Rat graft rejection models of corneal transplantationrnwere established. SD rats were receptor and Wistar rats were donors. After corneal allografts were performed, 48 SD rats were randomly divided into three groups, 16 rats in each group. Group A was blank control group; group B was treated by oculotect gel (containing vitamin A); group C was treated by 1g/Lrndexamethasone eyedrops. Besides, group D was normal unoperated eyes.Slit-lamp microscope was employed to record and compare rejection index (RI) of corneal transplantation. Through HE,PAS staining of conjunctival histological sections and image analysis system, the number and morphology of conjunctival goblet cells were observed and analyzed between operation group and normal group.·RESULTS: The HE, PAS staining detection showed that the number of conjunctival goblet cells in oculotect gel group,1g/L dexamethasone eyedrops group and control group is lower than that in normal group after surgery (P＜0.01). The number of conjunctival goblet cells in oculotect gel group and 1g/L dexamethasone eyedrops group is higher than that in control group (P＜0.05). The number of conjunctival goblet cells in 1g/L dexamethasone eyedrops group is higher than that in oculotect gel group (P＜0.05).·CONCLUSION: The results indicate that vitamin A may inhibit the decrease of conjunctival goblet cells after corneal allograft rejection in rats.
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