不同时间电针对急性脑出血大鼠炎性免疫反应的影响

摘要

Objectives: To discuss the effect at different time of electro - acupuncture on inflammatory immune of rat with acute intracerebral hemorrhage. Methods:The intracerebral hemorrhage model was induced by injecting Collagenase Ⅶ into caudate nucleus. 3h EA Group and 72h EA Group were performed at 3h and 72h after modeling. The expression of TNF - αt and MMP - 9 was detected in the rat brain. Results: Rats with intracerebral hemorrhage had an obvious higher expression of TNF - α and MMP - 9, which was coupled with temporal change to some degree. The expression of TNF - αand MMP - 9 were reduced in both 3h and 72h groups, but with obviously statistical difference ( P ＜ O. 05 ). Conclusions: Electro - acupuncture can lower the expression of TNF - α and MMP - 9 in brain. The 72h EA treatment has a more obvious effect than the 3h EA. Electro - acupuncture should be used prudently at early stage of acute intracerebral hemorrhage.%目的:观察不同时间点电针对急性脑出血大鼠炎性免疫反应的影响.方法:采用胶原酶Ⅶ诱发大鼠尾状核脑出血模型,分别在造模后3h、72h时开始针刺.检测各组脑组织TNF-α、MMP-9的表达.结果:脑出血大鼠TNF-α、MMP-9表达明显增高,并与时间变化有一定联系.3h、72h电针组TNF-α、MMP-9表达均降低,72h电针组较3h电针组低,有显著性差异(P<0.05).结论:电针可以抑制脑出血后诱发的炎性免疫反应,急性期脑出血早期应慎重选择电针治疗.