一种增强型荧光探针对Al3+的识别检测及细胞成像研究

摘要

Based on the inhibition of C=N isomerization and chelation-enhanced fluorescence (CHEF),the simple Schiff base 1-(2-pyridylmethyleneimino) naphthalen-2-ol (L) acts as an effective fluorometric sensor for Al3+ in buffered semi-aqueous medium.L exhibited selective fluorescence enhancement upon binding AP+ as a detection response,which giving strong blue emission (438 nm).The sensitivity of the fluorescent based assay (80.5 nmol/L) for Al3+ is far below the limit recommended in the World Health Organization (WHO) guidelines for drinking water (7.41 μmol/L).L was successfully applied to detect intracellular Al3+ in human SiHa cells through fluorescence microscopic imaging.The interaction between the sensor and Al3+ has been investigated in detail by NMR and HRMS analysis.%以2-羟基-1-萘甲醛和2-氨甲基吡啶为原料合成了一种简单的希夫碱化合物(L).L作为增强型荧光探针,在缓冲水溶液中可选择性检测Al3+,检测限为80.5 nmol/L,远低于世界卫生组织规定的饮用水中Al3+的上限值(7.41μmol/L).通过荧光共聚焦成像,化合物L可高效地检测人体SiHa癌细胞内的Al3+.