三七不同部位总皂苷溶血性及体外免疫活性研究

摘要

Panax notoginseng saponions (PNS) was purified by conventional butanol extraction and macroporous resin chromatography. Erythrocyte hemolysis rate was determined by hemdytic test, PNS biological immune activity from different parts of P. Notoginseng was measured by spleen lymphocyte proliferation test. Results showed that hemolytic characteristics of different parts of P. Notoginseng were smaller, and could significantly promote lymphocyte proliferation induced by ConA and LPS, and immune enhancement to the effect of total saponins of P. Notoginseng root was better. The study showed that the security of PNS from different parts of Panax notoginseng was good, and could promote cellular and humoral immunity.%通过常规正丁醇法提取,大孔树脂层析分离纯化得三七(Panax notoginseng)总皂苷;通过溶血试验测定红细胞溶血率;脾淋巴细胞增殖试验测定三七不同部位总皂苷的生物免疫活性.结果表明,三七不同部位总皂苷溶血性较小,且均能显著促进ConA和LPS诱导小鼠淋巴细胞增殖反应,其免疫增强作用以三七主根总皂苷的效果较好.表明三七不同部位总皂苷安全性好,能促进细胞免疫和体液免疫.