植物抗病WRKY转录因子生物信息学分析

摘要

以GenBank上登录的拟南芥(Arabidopsis thaliana)、欧芹(Petroselinum crispum)、辣椒(Capsicum annuum L.)、水稻(Oryza sativa L.)和毛白杨(Populus tomentosa)的22个WRKY家族抗病转录因子为分析对象,采用生物信息学方法对其系统发生树、保守基序和蛋白质三级结构进行分析。结果表明,22个抗病WRKY转录因子分为2个大类群,7个亚类群。两大类群的转录因子都具有基序1和2,系统发育树中第一大类群中的拟南芥AtWRKY4和AtWRKY25,欧芹PcWRKY1和辣椒CaWRKY-a具有相似的蛋白质三级结构,其都具有基序9和基序12,拟南芥AtWRKY33和AtWRKY48具有相似的蛋白质三级结构,都具有基序18；系统发育树中第四亚类群中的辣椒CaWRKY1和毛白杨PtWRKY23,具有相似的蛋白质三级结构。%The phylogenetic tree and conservative motif and tertiary structure of protein from 22 WRKY transcription factors with resistance to disease were analyzed in Arabidopsis thaliana, Petroselinum crispum, Capsicum annuum L. and Oryza sati-va L., Populus tomentosa, which registered in GenBank by bioinformatics methods. The results showed that 22 WRKY tran-scription factors with resistance to disease were divided into two main groups, seven sub-groups. They had the conservative motif 1 and 2. The tertiary structure of protein encoded by AtWRKY4 and AtWRKY25, PcWRKY1 and CaWRKY-a, be-longed to the first main group, was similar. They had the conservative motif 9 and 12. The tertiary structure of protein en-coded by AtWRKY33 and AtWRKY48, and they had the conservative motif 18. The tertiary structure of protein encoded by CaWRKY1 and PtWRKY23 was similar, and they belonged to the forth sub-group.