Isoelectric focusing (IFF) technique was used to locate biochemical loci in Agropyron elongatum by using wheat Agropy ron elongatum addition lines. There were six loci being located initially in all. The structural genes of Est E5 and Est E8 were located in 3EL, β Amy 1 in 4EL, Per E1 in 7E, and Per E4 in 5E. The α Amy E1 was relocated in 6EL. Chromosome location of these genes provide evidence of homoeology between wheat groups 3, 4, 6 and Agropyron elongatum chromosome 3E, 4E, 6E, respectively. It also indicated that chromosome rearrangement probably took place between 1E and 7E chromosomes during the evolution of the E genome.
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