阿司匹林抵抗与血小板膜GPⅡb/Ⅲa受体基因多态性的相关性

摘要

目的 探讨血小板糖蛋白血小板膜GPⅡb/Ⅲa受体基因多态性与阿司匹林半抵抗或抵抗的相关性.方法 连续服用阿司匹林100 mg·d-1共7d的老年冠心病患者200例.采用二磷酸腺苷(ADP)和花生四烯酸(AA)作诱导剂测定血小板聚集功能.采用聚合酶链反应-限制性核酸内切法(PCR-RFLP)检测PLA2/PLA1基因多态性.结果 阿司匹林半抵抗发生率为22.5％,阿司匹林抵抗发生率为16.5％,阿司匹林敏感发生率为61.0％.阿司匹林半抵抗和抵抗两组患者中PLA2等位基因出现的频率为11.54％,明显高于阿司匹林敏感组的患者组PLA2等位基因出现的频率(1.22％)(P＜0.05).结论 血小板膜GPⅡb/Ⅲa的PLA2等位基因与阿司匹林抵抗的发生相关联,可能为阿司匹林抵抗发生的一种遗传易感性标志.%Objective To explore the correlation between platelet membrane glycoproteina II b/ El a receptor gene polymorphism and aspirin semi-resistance or resistance. Methods A total of 200 elderly patients with coronary heart disease were administrated with aspirin at the dose of 100 mg · d-1 daily for7 days, and platelet aggregation function was measured with aden-osine diphosphate( ADP)and arachidonic acid(AA) as an inducer. PLA2/PLA1 gene polymorphism were detected via polymer-ase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results The semi-resistance, resistance as well as sensitivity of aspirin( AR, ASR and AS) were 22. 5% , 16. 5% and 61. 0% , respectively. The frequency of PLA2 allele gene in AR and ASR group was 11.54% , which was higher than the AS group (1.22% ) (P<0.05). Conclusion The PLA2 allele gene of the platelet membrane GP Ⅱ b/ Ⅲ a is associated with aspirin resistance, which may be a marker of genetic susceptibility to aspirin resistance.