复方一把针颗粒对原发性高血压大鼠高血压早期肾损伤的影响∗

摘要

目的：观察复方一把针颗粒对原发性高血压大鼠( SHR)的降压作用。方法取健康Wistar大鼠10只作为正常对照组；60只SHR按数字表法随机分为模型对照组，卡托普利组，珍菊降压片组，复方一把针大剂量组、中剂量组和小剂量组，每组10只。卡托普利组给予卡托普利30 mg•kg－1•d－1，珍菊降压片组给予珍菊降压片0.6片•kg－1，复方一把针大剂量组、中剂量组和小剂量组给予复方一把针13.18，6.59和3.3 g•kg－1•d－1，正常对照组和模型对照组灌胃给予等容积0.9％氯化钠溶液，连续8周。观察大鼠收缩压和舒张压的变化及血液中尿素、肌酐、一氧化氮( NO)、一氧化氮合酶( NOS)和血管紧张肽Ⅱ( AngⅡ)的影响。结果复方一把针大剂量组、中剂量组和小剂量组大鼠在给药2周后舒张压明显降低，复方一把针大剂量组给药4周后收缩压与舒张压明显降低，与模型对照组比较差异有统计学意义(P<0.05)；给药8周后复方一把针大剂量组、中剂量组和小剂量组尿素和肌酐含量明显低于模型对照组，差异有统计学意义( P<0.05)。复方一把针大剂量组大鼠NOS和AngⅡ的含量明显降低，NO含量升高，与模型对照组比较差异有统计学意义( P<0.05或P<0.01)。结论复方一把针颗粒减小SHR早期肾损伤作用与降低舒张压、尿素和肌酐、AngⅡ及调节NOS和NO水平有关。%Objective To observe the antihypertensive effect of compound Yibazhen granules on spontaneous hypertensive rats ( SHR) . Methods Wistar rats were served as normal control group. Sixty SHR were randomly divided into model control group,captopril group,Jane chrysanthemum antihypertension tablet group and compound high dose group,middle dose group and low dose group ( n = 10 each group ) by digital table method. Captopril group was given captopril 30 mg.kg-1 .d-1 ,and Jane chrysanthemum antihypertension tablet group was treated with Jane chrysanthemum antihypertension tablet ( 0. 6 tablet per kg ) , compound Yibazhen granules high dose group, middle dose group and low dose group received compound of 13.18,6.59 and 3.3 mg.kg-1 .d-1 ,respectively. Normal control group and model control group were intragastrically administered with 0.9% sodium chloride solution for 8 weeks. Changes of systolic and diastolic blood pressure of rats and blood urea,creatinine,nitric oxide (NO),nitric oxide synthase (NOS) and angiotensin Ⅱ (Ang Ⅱ) were observed. Results Diastolic pressure of rats in compound Yibazhen granules high dose group, middle dose group and low dose group decreased significantly in 2 weeks. Systolic blood pressure and diastolic blood pressure of compound Yibazhen granules high dose group decreased significantly in 4 weeks,compared with the model control group (P<0.05). Compared with the model control group, concentration of urea and crea in compound Yibazhen granules high dose group, middle dose group and low dose group were significantly lower( P<0.05) . The content of NOS and AngⅡ in rats of compound Yibazhen granules high dose group decreased significantly and the contents of NO increased, which were compared with the model control group ( P<0. 05, P<0. 01 ) . Conclusion The protective effect of compound Yibazhen granules in treating early renal damage in SHR is related to decreasing diastolic blood pressure,concentration of urea,crea and AngⅡ and regulating the levels of NOS and NO.