粉红粘帚霉67-1厚垣孢子形成与贮存研究

摘要

In order to develop Gliocladium biocontrol preparation ,taking Clonostachys rosea 67-1 chlamydospores as test materials ,the growth process ,morphology ,shelf life ,storage media ,water content and comparation between chla-mydospores and conidia were studied .The results showed that chlamydospores produced in specific medium after cul-turing 24 hours ,chlamydospores formed in the centre of hyphae or at the top ,and it could fall off into the medium . Circular or ellipse thick wall structure and incrassate cell wall could be seen under the electron microscope .In the same storage conditions ,chlamydospores had longer shelf life than condia .Under low temperature ,the storage medium of 10% water content could extend shelf life of chlamydospores of Clonostachys rosea 67-1 .%为研制粘帚霉生防制剂，以粉红粘帚霉67-1为材料，对粉红粘帚霉67-1厚垣孢子的生长过程、孢子结构、贮存介质和制剂含水量以及厚垣孢子和分生孢子的货架期比较进行了研究。结果表明：在特定的培养基中，粉红粘帚霉67-1在培养24 h后产生厚垣孢子，厚垣孢子形成于菌丝顶端或中部，并可以脱落游离到培养基中。电镜下可以看到圆形或椭圆形厚垣孢子以及加厚的细胞壁。相同贮存条件下，厚垣孢子的货架期比分生孢子长。低温条件下，10％含水量的贮存介质更利于厚垣孢子的贮存。