部分小麦种质中抗秆锈病基因 S r22的初步分子检测

摘要

Sr22 is an important stem rust resistant gene ,not only resistant to the domestic main stem rust races ,but al-so to the new virulent pathogen Ug99 (TTKSK) and its variants races TTKST and TTTKS .The microsatellite mark-ers closely linked to Sr22 were screened to be gene diagnose for resistant germplasm and varieties .Sr22 gene in wheat cultivars was identified by using a SSR marker Xcfa2019 ,the marker with good repeatability and high accuracy and could be therefore used for the identification of Sr22 in wheat breeding programs .The results indicated that 0 genotype carried the resistance gene among the 58 improved resistant Ug99 wheat cultivars from abroad and domestic ,and 3 genotypes carried the resistance gene among the 18 main cultivars of Heilongjiang ,with a frequency of 4 .0% .It reflec-ted that Sr22 occupied a certain proportion in Heilongjiang main plant cultivars and it should be continue to strengthen use of Sr22 in wheat breeding program in the future .%　　抗病基因 Sr22不仅抗国内流行的主要小麦秆锈病生理小种,而且对新型秆锈菌生理小种 Ug99(TTK-SK)及其变异种 TTKST 和 TTTKS 具有很好的抗性。利用与抗病基因 Sr22紧密连锁的 SSR 引物 Xc-fa2019,对从国内外搜集的抗 Ug99的小麦种质以及黑龙江省主栽小麦品种进行 SSR 分子检测。结果表明：从国内外引进的58份抗 Ug99小麦种质中未检测到抗病基因 Sr22；黑龙江省18份主栽春小麦品种中有3份材料中含有抗病基因 Sr22,占检测材料的4%。表明抗病基因 Sr22在黑龙江省主栽小麦品种中占有一定的比例,今后要继续加强 Sr22在小麦抗病育种中的应用。