目的:探讨松花粉预防用药对雄性衰老大鼠性腺轴分泌功能的影响.方法:选用8周龄雄性SD大鼠60只,体重180~220g,随机分为正常对照组、模型组、松花粉预防中(Pz)、低(Pd)剂量组,每组15只.除正常组外其余各组大鼠均采用D-半乳糖连续腹腔注射建立亚急性衰老大鼠模型,预防组在腹腔注射D-半乳糖的同时灌胃松花粉,用药50 d.检测各组血清胰岛素样生长因子-1(IGF-1)、黄体生成素(LH)、促卵泡激素(FSH)、睾酮(T)及下丘脑促性腺激素释放激素(GnRH)的含量.结果:模型组大鼠血清中LH、FSH及下丘脑中GnRH含量明显升高,IGF-1、睾酮含量明显下降,与正常组比较差异有显著性(P<0.05);松花粉中、低剂量组均能不同程度降低大鼠血清LH、FSH及下丘脑中GnRH含量,提高IGF-1、睾酮浓度,与模型组比较,差异显著(P<0.05).结论:松花粉可以预防雄性衰老大鼠性腺轴分泌功能紊乱,对雄性衰老大鼠性腺轴有较为明显的保护作用.%Objective: To approach the effect of pine pollen preventive medication on the secretion function of gonad axis in male aging rats. Method: Sixty rats of 8 weeks old male SD rats (weight: 180-220g)were randomly divided into 4 groups, 15 rats in each: the normal control group , the aging model group,the pine pollen middle dosage preventive group, the pine pollen low dosage preventive group. Except the normal control group, the rats of rest groups were all made by injecting D-gal into abdominal cavity continually. At the same time, the preventive group was made by intragastric administration pine pollen for 60 days. To detect the contents of IGF-1 ,LH,FSH,T in the blood and GnRH in the hypothalamus. Result: The contents of LH,FSH and GnRH in model group were obviously increased, and the content of IGF-1 and T in the model group were obviously decreased, compared with the normal group there was significant deviation(P＜0.05);the pine pollen middle,low dosage preventive group can decrease the contents of LH、 FSH in the blood and GnRH in the hypothalamus,and increase the contents of IGF-1 and T, compared with the model group there was significant diiference(P＜0.05). Conclusion: Pine pollen can prevent the secretion functional disorder of gonad axis in male aging rats, there is obvious protection on the secretion function of gonad axis in male aging rats.
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