宫颈癌及癌前病变中EZH2和PTEN蛋白的表达分析

摘要

Objective: To investigate the expression and significance of EZH2 and PTEN in the tissues of cervical carcinoma and cervical precancerous lesion. Method: The expressions of EZH2 and PTEN protein were detected by immunohistochemistry method in the tissues of invasive carcinoma of cervix utery and cervical precancerous lesion. Analysis the expression relationship between EZH2 and PTEN in the cervical carcinoma. Result: In normal cervical tissue, none of them has EZH2 expression and all of them has PTEN expression. The ratio of EZH2 expression in CIN and cervical carcinoma was 47. 5% and 75. 0% respectively, while PTEN expression in CIN and cervical carcinoma was 72. 5% and 15. 0% . The expression of EZH2 and PTEN protein in cervical carcinoma was differed significantly with histological differentiation, lymphatic metastasis and infiltration depth(P<0.05). The expression of EZH2 was negatively correlated with PTEN(P<0.05). Conclusion: EZH2 and PTEN may play an important role in the pathogenesis and development of cervical squamous cell cancer, and can be the indexes for estimating the level of malignant and progression of cervical carcinoma.%目的:分析宫颈癌及癌前病变组织中EZH2和PTEN的表达水平,探讨这两种蛋白在疾病发展过程中的意义.方法:使用免疫组化方法检测正常宫颈组织、宫颈癌及癌前病变组织中EZH2和PTEN的表达变化,分析两种蛋白的相关性及其在宫颈癌发生发展中的意义.结果:正常宫颈组织不表达EZH2,而PTEN均有表达;在宫颈上皮内瘤变及宫颈鳞癌组织中EZH2表达率分别为47.5%和75.0%,而PETN分别为72.5%和15.0%.EZH2和PTEN在不同分化、淋巴转移及浸润深度的宫颈癌组织中表达不同(P<0.05);EZH2与PTEN在宫颈鳞癌组织中具有负相关性(P<0.05).结论:检测EZH2和PTEN的表达水平,在评估宫颈上皮内瘤变及宫颈鳞癌的恶性程度及判断预后具有一定的意义.