吲哚胺2，3-双加氧酶的表达与宫颈癌发生和病理进程的关系研究

摘要

Objective To investigate the correlation between the expression of indolakylamine 2,3 dioxygenase (IDO)and the pathogenesis and pathological process of cervical cancer. Methods Seventy - five cases of stone sodium tissue specimens after cervical cancer surgery(cervical cancer group)were collected in Department of Gynaecology and Obstetrics of our hospital,and 30 cases of cervical intraepithelial neoplasia class I(CIN gradeⅠgroup),30 cases of cervical intraepithelial neoplasia class Ⅱ ~ Ⅲ(CIN gradeⅡ ~ Ⅲ group),30 cases of normal cervical tissues(control group),were collected in the study. The positive expression rates of IDO were detected by immunohistochemical method in all the groups,moreover,the correlation between the expression of IDO and pathological process of cervical cancer,survivorship and prognosis of patients was analyzed. Results The positive expression rates of IDO in cervical cancer group were significantly higher than those in CIN gradeⅠgroup,CIN gradeⅡ ~ Ⅲ group,control group(P < 0. 05). The positive expression rates of IDO in Ⅱ ~ Ⅲ group were significantly higher than those in control group( P < 0. 05). The positive expression rates of IDO in patients with poorly differentiated cervical cancer,patients with Ⅲ + Ⅳ stage cervical cancer,patients with lymph node metastasis,were obviously increased,furthermore,the positive expression rates of IDO were closely correlated to the differentiation degree of cervical cancer,FIGO staging and lymph node metastasis( P < 0. 05). After 36 - month follow up,among 53 patients with positive expression of IDO,7 patients died and 2 patients were failure to follow up,among 22 patients with negative expression of IDO, 3 patients died and 1 patient was failure to follow up. There was no significant difference in 3 - year mortality between patients with positive expression of IDO and patients with negative expression of IDO( P > 0. 05). The 3 - year survival neutral time in patients with negative expression was 35. 4 months,which in patients with positive expression of IDO was 34. 2 months,there was no significant difference between them( P > 0. 05). Conclusion The high - expression of IDO is observed in cervical cancer,which is correlated to differentiation degree of cervical cancer,FIGO staging ,lymph node metastasis at certain extent, however,which is not related with 3 - year survival prognosis of patients,it is need to lengthen follow up time to observe further.%目的：探讨吲哚胺2，3-双加氧酶（IDO）的表达情况与女性宫颈癌的发生与病理进程的相关性。方法选取宫颈癌手术治疗后的石钠组织标本75例（宫颈癌组），宫颈上皮瘤变Ⅰ级标本30例（CINⅠ级组）、宫颈上皮瘤变Ⅱ~Ⅲ级标本30例（CINⅡ~Ⅲ级组）、体检收集的正常宫颈标本30例（正常组），采用免疫组织化学法检测各组样本中的 IDO 阳性表达率，并追踪观察 IDO 表达与宫颈癌患者病理及生存与后的关系。结果宫颈癌组标本的 IDO 阳性表达率显著的高于 CINⅠ级组、CINⅡ~Ⅲ级组和正常组（ P <0.05）；CINⅡ~Ⅲ级组 IDO 阳性表达率显著的高于正常组（ P <0.05）。低分化宫颈癌患者、Ⅲ+Ⅳ期宫颈癌患者、发生淋巴结转移的宫颈癌患者的 IDO 阳性率表达显著的增高，IDO 阳性表达与患者的分化程度、FIGO 分期、淋巴结转移具有一定的关系（P <0.05）。经过36个月的随访，IDO 阳性表达53例患者中有7例死亡、2例失访，IDO 阴性表达患者22例中有3例死亡，1例失访，IDO 表达阳性与阴性患者的3年病死率差异无统计学意义（ P >0.05）；IDO 阴性表达患者的3年生存中位时间35.4个月与 IDO 阳性表达患者的34.2个月差异无统计学意义（ P >0.05）。结论 IDO 在宫颈癌组织中高表达，与宫颈癌的分化程度、FIGO 分期、淋巴结转移具有一定的关系，与患者的3年生存预后关系不显著，需进一步延长随访时间进行观察。