漆酶/ABTS介质系统对蒽醌染料的脱色效果

摘要

为提高蒽醌类染料废水的脱色率,以红芝(Ganoderma lucidum)发酵所得漆酶粗酶液与ABTS组成漆酶/ABTS介质系统对蒽醌染料活性艳蓝KN-R进行了脱色试验,分别考察不同pH、温度、ABTS加入量、酶加入量、金属离子等条件对活性艳蓝KN-R脱色的影响.结果表明:最优脱色条件为pH4.0,温度30℃,ABTS浓度10/μmol/L,酶液用量为30U/mL;此条件下对400mg/L活性艳蓝KN-R进行脱色反应60 min,脱色率达92%以上;漆酶/ABTS脱色活性艳蓝的动力学参数Km值为3556 mg/L,最大反应速率Vmax为91.7mg/(L·min).%The effects of pH, temperature, metal ion and dosage of ABTS and enzyme on the decolorization effect of anthraquinone dye KN-R were studied in the test of decoloration of anthraquinone dye KN-R with mediator system, which was composed of laccase from Ganoderma lucidum and ABTS, to enhance decoloration rate of anthraquinone dye wastewater. Results showed that The optimal conditions included pH 4.0, temperature 30℃, ABTS concentration 10 μmol/L and laccase 30U/mL. the decoloring rate for 400 mg/L KN-R was over 92％ in 60 minutes under the optimal conditions. The Km of anthraquinone dye KN-R with laccase/ABTS was 3 556 mg/L and Vmax Was 91. 7 mg/(L. min).