缺磷对水稻幼苗活性氧代谢及抗氧化酶活性的影响

摘要

为了探明缺磷水稻根系保护酶活性与活性氧代谢的关系,为水稻高产提供理论依据,采用水培法,研究缺磷24 h和48 h对水稻幼苗活性氧代谢及抗氧化酶活性的影响.结果表明:1)在缺磷处理下红良优166和Ⅱ优3027的总根长度和活性氧含量增加.2)红良优166的超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)的活性持续上升,在缺磷48 h时达最高；Ⅱ优3027的SOD活性在缺磷48 h时最高,POD和CAT活性在缺磷24 h时最高,随后下降.因此,缺磷可促进水稻根系生长,诱发氧化胁迫产生,抗氧化酶活性的上升即是对缺磷胁迫的一种应答.%A solution culture experiment was conducted to investigate the effects of phosphorus deficiency on active oxygen metabolism and antioxidant enzyme activity in rice seedlings,so as to explore the relationship between protective enzyme activity and active oxygen metabolism.Results:1) The total root length and active oxygen content both in Hong liangyou-166 and Ⅱ you-3027 increased under phosphorus deficiency.2) The superoxide dismutase (SOD),peroxidase (POD) and catalase (CAT) activity in roots of Hong liangyou-166 continued to increase,and reached the maximum for 48 h.The SOD activity in roots of Ⅱ you-3027 reached the maximum after phosphorus deficiency treatment for 48 h,POD and CAT activity reached the maximum for 24 h.The results suggested that phosphorus deficiency promoted the root growth and induced the oxidative stress,and the increase of antioxidant enzyme activity was in accordance with defense response to phosphorus deficiency.