芦竹的组培快繁技术研究

摘要

In order to find out the most efficient way for rapid propagation of Arundo donax,replanted at Guangxi Insti?tute of Botany from Hungary,by testing different concentrations of plant growth regulators,and stems with auxiliary buds were used as explants. The results showed that the most suitable medium for the sprouting of auxiliary buds was MS+ 6?BA 4.0 mg·L?1+sucrose 30 g·L?1+agar 3.5 g·L?1,the germination rate could get to 90.0%. MS+ 6?BA 8 mg·L?1+IBA 0.8 mg·L?1+ sucrose 30 g·L?1+ agar 3.5 g·L?1was the most efficient proliferation medium, and buds were robust. The average proliferation rate was 4.3/30 d. Three or five sprouts linked together was found to be a better basic material for subculture in that they demonstrated a higher multiplying capacity.The most suitable rooting medium was 1/2MS+NAA 0.2 mg·L?1+sucrose 20 g·L?1+activated carbon 1 g·L?1with the five cm buds,in which the rate of roo?ting was 100% for 7 d,the average root number was four,and the length of the roots was 2-4 cm.The rooted seedings, which with roots 2-3 cm,were transplanted from culturing seedlings to fine river sand,yellow soil or humus soil after one week?day's treatment,the survival percent reaches 98%after a month.The current annual yield was about 5 500 kg on one acre by regular management in the experimental field.The research provides technical support for industry appli?cation of the improved Arundo donax preliminarily.%该研究以从匈牙利引进的良种芦竹带腋芽茎段为外植体,以MS为基本培养基,通过设计不同的生长调节剂配比,对芦竹腋芽诱导、继代增殖及生根培养基进行了研究.结果表明:良种芦竹初代诱导最佳培养基为MS+ 6?BA 4.0 mg·L?1+蔗糖30.0 g·L?1+琼脂3.5 g·L?1,萌发率为90.0%;继代增殖最佳培养基为MS + 6?BA 8.0 mg·L?1+ IBA 0.8 mg·L?1+ 蔗糖30.0 g·L?1+ 琼脂3.5 g·L?1,芽健壮均匀,增殖系数为4.3/30 d,基部保留3~5个芽作为继代培养物增殖效果最佳;选择高度5.0 cm以上的植株进行生根,最佳生根培养基为1/2MS + NAA 0.2 mg·L?1+ 蔗糖20.0 g·L?1+ 活性碳1.0 g·L?1,不添加琼脂,培养1周生根率为100.0%,平均生根4条,根长2.0~4.0 cm;将所得生根苗炼苗1周,以河沙、黄泥或腐质土作为栽培基质,移栽于阴蔽度70的大棚中,1个月后移栽成活率≥98.0%;移栽于实验田中,按常规方式进行管理,当年亩产量约为5500.0 kg.该研究结果为良种芦竹的大规模产业化应用提供了技术支撑.