两种浓度臭氧对实验兔股动脉和股静脉影像学及病理学的影响

摘要

目的：观察两种浓度的臭氧（O3）对实验兔股动脉和股静脉影像及病理学的变化，以评估臭氧对股动脉和股静脉的氧化程度。方法20只实验兔随机分为A、B两组。给予氯胺酮30 mg/kg肌内注射麻醉后，随机选择一侧股动脉和股静脉腹股沟处，在神经刺激器引导下，注射臭氧-氧气混合气体A组浓度50μg/mL 5 mL，B组浓度30μg/mL 5 mL。注射后2 h在实验兔耳缘静脉建立通道，给予氯胺酮10 mg/kg静脉注射麻醉后，再静脉注射碘佛醇2 mL/kg，立即行CT扫描（TOSHIBA AQUILION MULTI）。随后处死实验兔，取注射点腹股沟处股动脉和股静脉2 cm作病理学光镜检查。对侧相应部位作为空白对照。结果与空白对照比较，A、B两组的CT扫描结果显示，注射点腹股沟处股静脉显影正常，未见狭窄或扩张；A、B两组的股静脉直径差异无统计学意义（P>0.05）。50倍和100倍光镜结果显示，注射点腹股沟处股动脉、股静脉结构正常，血管壁完整。结论在股动脉和股静脉旁注射臭氧进行镇痛治疗是安全的。%ObjectiveTo observe the iconographical and pathological changes of femoral artery and vein in experimental rabbits after injection of ozone(O3)in two concentrations.MethodsTwenty experimental rabbits were randomized into either group A (n=10)or Group B (n=10). All rabbits were given intramuscular ketamine 30mg/kg, then mixed O3-O2 5 mL was injected in one randomized side of groin nearby femoral artery and vein under neurostimulator guidance. The concentrations of O3 in Group A and B were 50 μg/mL and 30 μg/mL respectively. Venous access was eatablished through auricular vein 2 h after O3 injection and ketamine 10 mg/kg followed by ioversol 2 mg/kg were given, then CT scan(TOSHIBA AQUILION MULTI) was performed. The rabbits were executed and femoral artery and vein 2 cm in injection site were taken for pathological analysis under light microscope, and the contralateral counterparts were taken as no-treatment control.ResultsCompared to the no-treatment control, the CT scan indicated that the visualization of femoral artery and vein in injection site were normal, and no any stenosis or ectasia was found. There was no signiifcant difference in the diameter of femoral vein between the two groups(P>0.05). The analysis under 50×or100×light microscope also showed a normal structure and intact vessel wall of femoral artery and vein in injection site.ConclusionIt is safe to inject O3 nearby femoral artery and vein for analgesia therapy.