MDM2启动子309位点多态性与稽留流产关系的研究▲

摘要

Objective To explore the association between single nucleotide polymorphism (SNP) of murine double minute 2(MDM2)-309 and missed abortion.Methods Genotyping of MDM 2 309 polymorphism was conducted by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) with villous samples collected from 40 women confirmed as missed abortion (study group) and from 40 healthy women with early pregnancy (control group). Results Calculation was performed following a dominant genotype model ,and we found that T/T genotype accounted for 27.50%(11/40) in the study group,and T/G plus G/G genotypes accounted for 72.50%(29/40),wherase in the control group,these figures were 35.0%(14/40) and 65.00%(26/40),respectively.No significant difference was found between two groups in the distribution of genotypes (P>0.05).When calculation was performed based on a recessive genotype model,we found that G/G genotype accounted for 32.50%(13/40) in the study group,and T/T plus T/G genotypes accounted for 67.50%(27/40),wherase in the control group,these figures were 12.50%(5/40) and 87.50%(35/40),respectively.There was a significant difference in the distribution of genotypes between two groups (P<0.05).Conclusion The MDM2 SNP309 G/G genotype is associated with an increased risk of missed abortion.Further study on its molecular mechanism is warranted .%　　目的探讨鼠双微体基因2（MDM2）启动子309位点单核苷酸多态性（SNP）与稽留流产的关系。方法对40例稽留流产妇女（研究组）的绒毛标本进行聚合酶链反应限制性片段长度多态性分析（PCR－RFLP），与40例健康早期妊娠妇女（对照组）的绒毛标本进行PCR－RFLP对照。结果根据显性基因模型计算，研究组T／T型占27．5％（11／40），T／G型＋G／G型占72．5％（29／40），对照组分别为35．0％（14／40）、65．0％（26／40），两组基因型分布比较，差异无统计学意义（P＞0．05）。根据隐性基因模型计算，研究组G／G型占32．5％（13／40），T／T型＋T／G型占67．5％（27／40），对照组分别为12．5％（5／40）、87．5％（35／40），两组基因型分布比较，差异有统计学意义（P＜0．05）。结论MDM2 SNP309 G／G基因型与稽留流产的发病风险增加有关，其确切的分子机制有待进一步研究。