抗阻力运动对大鼠股四头肌PI3K和Akt基因表达的影响

摘要

To explore the influence of the moderate intensity resistance training on the expression of PI 3K and Akt gene in skeletal musle .Methods: 18 healthy Sprague -Dawlev rats were randomly distributed into three groups:normal control group ( NC, n =6 ); aerobic exercise group ( AE, n =6 ); medium resistance training group (MRT, n=6).Quadriceps femoris muscles were excised after 8 weeks training and freezed in liquid nitrogen and analyzed by reverse transcription PCR .Results:After 8 weeks resistance training , the expression of PI3K mRNA in medium resistance training rats was significantly different from that in normal control group and aerobic exercise group rats (p<0.05).And the expression of Akt mRNA has no significant difference between resistance training group and aerobic exercise group rats (p>0.05), but medium resistance training group was markedly higher than normal control group in the expression of Akt mRNA (p<0.05).Conclusion:The expression levels of PI3K mRNA and Akt mRNA in quadriceps femoris muscles can be improved by resistance training and aerobic exercise . It conjectures that treadmill resistance exercise can strengthen the signal transduction of PI 3K/Akt signal pathway , resulting in promote glycogen and protein synthesis .%探讨中等强度抗阻力训练对骨骼肌PI3K基因和Akt基因表达的影响。方法：18只健康的SD大鼠随机分为安静组、有氧运动组和抗阻力训练组，每组6只。训练组采用Bedford等人跑台训练方案。8周训练结束后将大鼠安静状态下处死，取股四头肌，液氮冻存。采用Reverse Transcription -PCR方法测定大鼠股四头肌PI3K mRNA和AKt mRNA表达。结果：8周训练后，抗阻力运动组大鼠股四头肌PI3K mR-NA的表达较安静对照组和有氧运动组显著增多（ p＜0．05）。 Akt mRNA表达较有氧运动组无显著差异（p＞0．05），较安静对照组有显著增长（p＜0．05）。结论：抗阻力跑台运动和有氧运动均可促进大鼠股四头肌PI3K mRNA和Akt mRNA表达，推测抗阻力运动可增强PI3K/Akt信号途径中信号转导，对促进糖原和蛋白合成有促进作用。