饲料中添加谷胱甘肽对吉富罗非鱼抗氧化和抗亚硝基氮应激能力的影响

摘要

选择初始体重(3.27 ±0.04)g的吉富罗非鱼(GIFT Oreochromis niloticus),分别投喂基础饲料和添加80、160、240、320和400 mg/kg谷胱甘肽(GSH)的试验饲料,记作GO、G80、G160、G240、G320和G400.49 d后,G320血清和G160、G240肝脏谷胱甘肽S-转移酶,G160、G240血清和G320肝脏谷胱甘肽还原酶,G240肝脏超氧化物歧化酶,G320血清和G240肝脏过氧化氢酶活性,G240～G400血清和G240肝脏总抗氧化能力,G320血清抗超氧阴离子活性,与对照组相比分别显著升高.G80～G320肝脏丙二醛含量显著低于对照组.在亚硝基氮应激96 h内,G320累积死亡率显著降低.结果表明,饲料中添加一定量的GSH能显著提高罗非鱼的抗氧化性能和抗亚硝基氮应激能力.%A feeding trial was conducted to investigate the effects of dietary glutathione ( GSH) on the antioxidant capability and resistance to nitrite exposure of GIFT Oreochromis niloticus. The fish, with an initial weight of (3. 27±0. 04) g, was fed a basal diet and five diets added with 80, 160, 240, 320, 400 mg/kg GSH respectively, named GO, G80, G160, G240, G320 and G400. After 49 days' feeding, it was found that glutathione S-transferase in serum of G320 and liver of G160 ~ G240, glutathione reductase in serum of G160 ~ G240 and liver of G320, superoxide dismutase activities in liver of G240, total antioxidant capacity in serum of G240 ~ G400 and liver of G240, anti-superoxide activities in serum of G320, were significantly higher than those of GO. Malondialdehyde content in liver of G80 ~ G320 significantly decreased in comparison with GO. Accumulative mortality rate under nitrite exposure for 96 h in G320 was significantly lower than GO. In conclusion, dietary GSH could improve the antioxidant capability and resistance to nitrite exposure of GIFT 0. niloticus.