患病细鳞鱼杀鲑气单胞菌的分离与鉴定

摘要

从患病细鳞鱼（Brachymystax lenok）的病变组织处分离到1株致病菌，经过分离培养，生化鉴定，16 S rRNA 序列分析和人工感染实验确定该病原菌为杀鲑气单胞菌无色亚种（Aeromonas salmonicida subsp．achromoge－nes）。采用20种药物进行药敏分析，结果显示：分离菌株对阿米卡星、哌拉西林、恩诺沙星等9种抗生素敏感；对环丙沙星、诺氟沙星等4种抗生素中度敏感；对卡那霉素、青霉素、氨苄西林等7种抗生素耐药。%The pathogenic strain was isolated from diseased Brachymystax Lenok.Diagnosis was based on staining micros -copy, biochemical identification, 16SrRNA sequence analysis and animal experiment .The isolated bacteria was identified as Aeromonas salmonicida subsp achromogenes.The drug sensitivity test of a total of 20 antimicrobial drugs Showed that the isolated strain was sensitive to 9 kinds of drugs including amikacin , piperacillin, enrofloxacin, etc., and moderately sen-sitive to ciprofloxacin, norfloxacin etc.The strain was resistant to 7 kinds of antibiotics including kanamycin , penicillin and ampicillin etc.