The in vitro plantlet leaves and stems of 4 elite Eucalyptus clones commonly cultivated in production were used as explants to study the callus induction and differentiation under different callus induction time, in order to establish an effective callus regeneration system. The results showed: The clones Eg5 and DH201-2 leaf callus inducted for 45 d were suitable for differentiation of adventitious buds, with the highest regeneration rate of 70.0% and 63. 3% respectively. 20 d and 15 d were the best induction times for leaf callus inducting and stem callus inducting of clone GL9, with the regeneration rates of 66.7% and 63. 3% respectively. The best regeneration rate of DH3229 was 16.7% from stem callus inducted for 30 d. There were significant differences in regeneration rate among the 4 clones. Clones Eg5, DH201-2, and GL9 were easy to regenerate compared with DH3229. No obvious difference was found in regeneration rate of leaf callus and stem callus from same clone.
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