天竺葵叶片愈伤组织诱导的研究

摘要

试验采用盆栽天竺葵的叶片为外植体，以MS为基本培养基，研究用不同浓度的6-BA和24-D配比组合及叶片大小对愈伤组织诱导的影响。结果表明,适宜天竺葵叶片诱导愈伤组织的最佳培养基为：MS +0.5 mg/L 2.4-D +0.05 mg/L 6-BA，愈伤组织诱导率较高，高达93.3%；叶片大小为0.5cm×0.5cm，能较好的促进愈伤组织的诱导，且污染率较低。%Plant hormones to cal us induction, the effects of the current domestic and overseas scholars to become the focus of research, with the constant expansion of its study field and the deepening of the research methods, plant hormones in scientific research, crops planted, vegeta-bles, flowers in the field of cultivation, used more widely. In the study, the potted geranium leaves for explant, MS with as the basic culture medium, with different concentration of cytokinins (6-BA) and 2 4-D the cal us induction study. The results show that:to 0.5 mg/L of 2 4-D and 0.05 mg/L of 6-BA of combination of auxin geranium blade cal us induction effect is best, cal us induction rate is higher, as high as 93.3﹪, namely the appropriate geranium blade cal us induction organization for the best medium:MS+2 4-D0.5 mg/L+6-BA0.05 mg/L.