Effect of Liposome-Encapsulated Total Alkaloid of Harmaline on Rabbit Lens Epithelial Cells:Experimental Study on the Prevention of Posterior Capsule Opacification

摘要

＜正＞ Purpose: To investigate whether liposome encapsulated total alkaloid of Harmaline （TAH） as a therapeutic agent is beneficial to prevention of posterior capsular opacifi-cation （PCO）.Methods: Liposome-encapsulated TAH was prepared by modified freeze-thawing method. 0. 1ml of liposome-encapsulated TAH （0. 2mg/ml） was injected into the capsular bag during extracapsular lens extraction （ECLE） of each eye in total 10 rabbit eyes. Blank liposome or balance salt solution （BSS） was used as control. Slit-lamp examination and histopathological examination was used to evaluated capsule opacifica-tion. Intraocular pressure （IOP） , density and morphology of corneal endothelia cells, the amplitude and latency of b wave of ERG were measured.Results: The inflammatory response was mild both in TAH treated and the control group. PCO formation occurred in the control group 2 weeks postoperatively, but the posterior capsule was clear in TAH treated eyes. 4 weeks and 8 weeks after operation, PCO occurred both in TAH treated