金属钴、铬离子对成骨细胞增殖及I型胶原蛋白表达功能的影响

摘要

Objective To investigate the cytotoxicity of cobalt (Co2+) and chromium (Cr3+) ions on mice osteoblast (MC3T3-E1) and the effects on the secretion of collagen-I from osteoblast under the stimulation of Co2+and Cr3+ions. Methods The osteoblast was cultured in vitro. The cell vitality was assured by MTT test. The ELISA and RT-PCR methods were applied to detect the col-lagen-I on gene and protein levels at 24 and 48 hours after co-culture respectively. Results Compared to the control,MTT test demonstrated that Co2+and Cr3+ions can obviously decrease the cell viability of osteoblast. When the osteoblasts were exposed to Co2+and Cr3+ions for 24 and 48 hours,the releasion of collagen-I decreased by 45.3% and 49.2% as compared with the control (P<0.05);the expression of collagen-I mRNA decreased by 26.8% at 24h,and 32.6% at 48h as compared with the group A (P<0. 05). Conclusion Co2+and Cr3+ions can inhibit the expression and release of collagen-I from osteoblast,and may have a cytotoxic effect on osteoblasts.%目的：观察钴、铬离子对小鼠成骨细胞(MC3T3-E1)的细胞毒性及I型胶原蛋白合成的影响，探讨钴、铬离子对骨代谢功能的影响机制。方法钴、铬离子分别与小鼠成骨样MC3T3-E1细胞体外培养，噻唑蓝(MTT)法测定细胞活力。 ELISA法检测培养上清液中I型胶原蛋白含量、RT-PCR检测成骨细胞I型胶原蛋白mRNA的表达。结果 MTT结果显示，与对照组相比，钴、铬离子能明显抑制成骨细胞的细胞活力；成骨细胞暴露在钴铬离子下，与对照组相比，24h、48h后：I型胶原蛋白的分泌分别下降45.3%、49.2%（P<0.05）；I型胶原蛋白mRNA表达分别下降26.8%、32.6%（P<0.05）。结论钴、铬离子可抑制成骨细胞I型胶原蛋白的分泌及其mRNA的表达，提示其对MC3T3-El细胞可能存在细胞毒性。