经过野外采样、稀释平板法、富集培养等步骧进行初步筛选得到12株菌,并通过测定其对NH4+-N去除效果,复筛得到1株对NH4+-N去除率最高的菌株,命名为Y8.该菌株为革兰氏阴性杆状菌.通过小试的初步研究,考察了不同碳源、温度、pH值、摇床转速对Y8去除NH4+-N的影响,实验结果表明:Y8在以丁二酸纳为唯一碳源、温度30℃、pH值9.5、转速150 r/min条件下去除NH4+-N效果最好；并且在有氧情况下对NO3-N和NO2--N均有一定去除效果.%12 strains were selected by some steps such as field sampling, convention plating and accumulation culture and a strain with the highest removal rate on NH+-N was repeated screened from the 12 ones by measuring the removal efficiency of NH4+-N, named Y8. The bacterium is Gram-negative and rod-shaped. Different carbon sources, temperature, pH and rotating speeds of cradle were investigated through batch test to optimize conditions for nitrogen removal. The results showed that Y8 has the highest removal rate for NH/-N when the single carbon source is sodium succinate, the temperature is 30 ℃, pH is 9.5 and cradle rotating speed is ISO r/min. Furthermore, Y8 has favorable removal efficiency of NO3---N and NO2--N in aerobic circumstance.
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