The collection of benthic amphibian in deepwater rivers is complicated, and the results are often different from the objective realities.The 81 sets of valid datum were obtained through investigation and monitoring of 11 fracture surfaces, 15 points in Heilong River, Songhua River and Wusuli River during 2012-2015, and the results, summarized and researched by statistical method, showed that there were significant differences in the number of species, community composition and evaluation results between different methods in the same sampling point and it was suggested that the aquatic ecology application of deepwater with the evaluation of benthic amphibian should be combined with the actual situation of sampling points,trying to use a variety of methods for the collection of benthic animals, and as a result, the combined statistics datum was more accurately representative to the objective realities of the collected points which was the premise of an objective and accurate assessment of water ecological environment.%深水河流底栖动物的采集比较复杂,采集结果与客观实际往往存在较大的差异,通过2012年-2015年对黑龙江、松花江和乌苏里江等11个断面15个点位的调查、监测,取得81组有效数据,利用统计学方法总结、研究,发现同一采样点不同方法之间的物种数、群落组成和评价结果均存在显著的差异,建议深水河流在应用底栖动物开展水生态评价时,应结合采样点的实际情况,尽量采用多种方法进行底栖动物的采集,合并统计的数据更能准确的代表采集点位的客观情况,是客观、准确评价水生态环境状况的前提.
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