HPLC法测定蒲参胶囊中2，3，5，4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的含量

摘要

Objective:To establish an HPLC method of determining 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside in Pushen Cap-sules.Methods:Agilent C18 column(4.6 mm ×250 mm, 5 μm) was used,the mobile phase was acetonitrile-water (17∶83) and the detection wavelength was 320 nm.Results:The linear range of 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside was 0.0320-0.960 6 μg( r=1.000 0) ,the average recovery was 96.6%with RSD 1.3%( n=6).Conclusion:The method is reliable,accurate and can be used to the quality control of the preparation.%目的：建立蒲参胶囊中2，3，5，4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷含量的HPLC测定方法。方法：采用Agilent C18柱（4.6 mm ×250 mm，5μm），流动相为乙腈-水（17∶83），检测波长为320 nm。结果：2，3，5，4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷在0.0320～0.9606μg范围内呈线性关系（r＝1.0000），平均回收率为96.6％（n＝6），RSD为1.3％。结论：所建方法简便、准确，可用于该制剂的质量控制。