The Study of Enzymology Characterization of Ctenopharyngodon idellus LDH Isozyme

摘要

LDH-A_4,B_4,and C_4 were isolated and purified from Ctenopharyngodon idellus tissues by means ofaffinity chromatography.The purity,amino acid content and kinetics were analyzed.The anti-LDH-A_4,B_4,and C_4 antibodies were prepared from rabbit sensitized by LDH-A_4,B_4,and C_4respectively.The LDH patterns of some freshwater fish and marine fish were identified by theantigen-antibody precipitation reaction.The homology between LDH isozyme molecules fromdifferent sources and the immunological homology of different LDH isozymes were discussed.