Effect of Thyroid Hormone Deficiency on Tubulin Synthesis in Developing Chick Brain

摘要

Thyroid hormone deficiency was created artificially in chick embryos by injecting propyl-thiouracil(PTU).The tubulin was quantified by ~3H-colchicine assay(expressed as colchicine bindingactivity).Thyroid hormone deficiency resulted in 16％ to 28％ decline of the tubulin level in thedeveloping chick brain from 15 to 19 days of embryonic age as compared with the control,whereasthe total protein amount was not significantly affected.It suggests that thyroid hormone specificallyaffected the synthesis of brain tubulin.