Background:Axonal degeneration caused by damage to the optic nerve can result in a gradual death of retinal ganglion cells(RGC),leading to irreversible vision loss.An example of such diseases in humans includes optic nerve degeneration in glaucoma.Glaucoma is characterized by the progressive degeneration of the optic nerve and the loss of RGCs that can lead to loss of vision.The different animal models developed to mimic glaucomatous neurodegeneration,all result in RGC loss consequent optic nerve damage.Methods:The present article summarizes experimental procedures and analytical methodologies related to one experimental model of glaucoma induced by optic nerve crush(ONC).Point-by-point protocol is reported with a particular focus on the critical point for the realization of the model.Moreover,information on the electroretinogram procedure and the immunohistochemical detection of RGCs are described to evaluate the morpho-functional consequences of ONC.Discussion:Although the model of ONC is improperly assimilated to glaucoma,then the ONC model simulates most of the signaling responses consequent to RGC apoptosis as observed in models of experimental glaucoma.In this respect,the ONC model may be essential to elucidate the cellular and molecular mechanisms of glaucomatous diseases and may help to develop novel neuroprotective therapies.
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机译:Structure Determination and Mechanistic Insights of: I.Cyanobacteriochrome NpR6012g4 Light Sensor Protein in Phototaxis II.Retinal Degeneration 3 (RD3) Protein in Vision III.Ryanodine Receptor 2 (RyR2) Regulation by Calmodulin (CaM) in Cardiac Function =结构测定和机理洞悉:I.趋光性中的蓝细菌色素NpR6012g4光敏蛋白 II.视觉作用中的视网膜退化蛋白3 III.心脏功能中的钙调蛋白调控兰诺定受体2