Objective: Breast Cancer (BC) is characterized by high complexity and heterogeneity, and microRNA (miRNA) is bound up with the occurrence and development of BC. In this study, we evaluated the prognostic value of miRNA in BC. Background: Breast ductal and lobular cancers are the most common types of Breast Carcinomas (BC) and indicate the high complexity heterogeneity in this disease. Each BC patient has unique morphological and molecular features. MicroRNAs (miRNAs) play a critical role in human oncogenesis, progression, and prognosis. Our study aimed to identify potential prognostic biomarkers of breast ductal and lobular cancers to predict the overall survival outcome. Methods: All analyzed miRNA sequencing and clinical data were obtained from the Genomic Data Commons Data Porta. edgeR package in R software was used to analyze the differential miRNA expression profiles. Complete survival information and differentially expressed miRNA expression were obtained and the Caret package was used for random division of the samples along with their profiles into two groups (training group and test group). We performed univariate Cox regression analyses for miRNAs in the training group. We utilized three different web-based tools to identify the target genes of miRNAs and used the Perl language to evaluate the target genes for miRNA signature. STRING database was used to assess PPIs. Results: A total of 304 differentially expressed miRNAs were identified (213 were upregulated and 91 were downregulated). Among these, nine (hsa-miR-204-5p, hsa-miR-7706, hsa-miR-1247-3p, hsa-miR-20b-3p, hsa-miR-605-5p, hsa-miR-615-3p, hsa-miR-4652-5p, hsa-miR-9-5p, hsa-miR-2115-5p) miRNAs which remarkably correlated with overall survival rate of BC were performed by Cox regression analysis and miRNA signature risk score built. And then we performed the model of BC patients for three years survival risk, the AUCs of ROC were 0.804, 0.667, and 0.739 in the training, test, and entire groups, respectively. miRNAs were differentially expressed in tumor-related biological processes and pathways by functional enrichment and bioinformatic analysis. Conclusion: The current study provided novel insights into the miRNA-based mRNA network in BC. The nine miRNA and ten hub genes may be independent prognostic signatures for survival prediction in BC patients.
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机译:Data mining of Arabidopsis thaliana salt-response proteins based on bioinformatics analysis Guo Meili, Gao Weixi, Yu Xuejuan, Zhou Chunxi, Liu Fujun, Liu Xin Abstract
机译:Retraction statement: ‘Glabridin attenuates the migratory and invasive capacity of breast cancer cells by activating microRNA‐200c’ by Xianqing Ye, Fei Jiang, Yuan Li, Juan Mu, Lu Si, Xingxing Wang, Shilong Ning and Zhong Li