Reversal of acetaminophen-generated oxidative stress and concomitant hepatotoxicity by a phytopharmaceutical product

摘要

The increasing popularity of herbal medicine and the well-established health benefits of phytochemicals have spurred the multiplicity of nutraceutical and phytopharmaceutical products.In this study,Trévo^TM,a nutraceutical and phytopharmaceutical product,was evaluated for beneficial effects in acetaminophen-induced hepatic toxicity in Wistar rats.Animals received Trévo^TM(1.5 mL/kg,3.0 mL/kg or 4.5 mL/kg)orally for 14 days.Hepatotoxicity was induced by the oral administration of acetaminophen(2 g/kg),24 h prior to sacrifice.Biochemical liver function tests,oxidative stress indicators and histoarchitectural changes were evaluated.Acetaminophen administration occasioned significant increase(P<0.05)in serum bilirubin level and activities of the aminotransferases,alkaline phosphatase,γ-glutamyltransferase and lactate dehydrogenase accompanied by a significant decrease(P<0.05)in albumin level as well as histopathological alterations in liver sections.Promotion of hepatic oxidative stress by acetaminophen was revealed by significant(P<0.05)increase in lipid peroxidation,depletion of reduced glutathione,and decrease in superoxide dismutase and catalase activities.Administration of Trévo^TM remarkably ameliorated acetaminophen-induced histopathological alterations and changes in serum and tissue biochemical markers.The protective effect of Trévo^TM(4.5 mL/kg)was at par with that of Silymarin(25 mg/kg).The present study indicates that Trévo^TM has notable salubrious effects.