Faba bean (Vicia faba L) seeds are an important source of plant protein for humans and animals. A total of 15,697 Differentially Expressed Genes (DEGs) with pathway annotation were discovered in RNA-Seq of the faba bean seeds. A total of 75 significant KEGG pathways abundance were discovered and 9 pathways were conserved within all genotypes. 41 significant pathways were found to be partially conserved within comparisons of 2 to 6 pairs of genotypes and 25 significant pathways were unique to single pairs of genotypes. There were 8 specific significant pathways discovered related to the faba bean seed Hydration Capacity trait and 9 specific significant pathways discovered related to the PSbMV seeds staining trait. The DEGs demonstrated the genetic distance between these varieties was confirmed by the breeding pedigree selection information and a PCA graph clearly illustrated the genetic distance within these genotypes.
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