Use of Isoproturon Imprinted Polymer Membranes as a Selective Recognition Platform in a Resistance Based Electrochemical Sensor

摘要

Methacrylic acid with isoproturon has been utilized to prepare molecularly imprinted polymeric (MIP) membrane on Whatman filter paper no. 5 for selective electrochemical estimation of isoproturon pesticide. MIP membrane was prepared by radical polymerization and characterized using Ultra Violet spectrophotometer, Fourier Transform Infra Red (FTIR) spectroscopy and Scanning Electron Microscopy (SEM). Contact angle study was used to determine the surface energy of the MIP membrane and electrical conductivity measurements were performed by applying a small-amplitude alternating voltage (20 mV) with frequencies varying from 20 Hz to 80 kHz generated by a low-frequency wave form generator. Different isoproturon concentrations in samples were tested and analyzed. Results indicate linear increase in membrane resistance with increasing isoproturon in 10-3 to 10-6 M range. The selectivity of the electrochemical sensor was confirmed by testing isoproturon in presence of the structurally related compounds monouran and diuran. Results reveal highly selective and sensitive sensor, which can be employed for regular estimation of isoproturon in fields.