Aim: In this in vitro study the variation of root anatomy and canal system of the first human maxillary premolar was evaluated. Materials and Methods: Two hundred and twenty one maxillary first premolars (#221 teeth) were examined. All of the teeth were identified using the accepted criteria of Woelfel as maxillary first premolars. Selected teeth are analyzed using operating microscope (OP) and computed dental radiography (CDR). Results: There was a higher incidence of two-roots form 70.14% (n = 155), 21.72% (n = 48) had one root, and 8.14% (n = 18) had three roots. In the two-root category, 16.29% (n = 36) had bifurcation on apical third, 21.72% (n = 48) had bifurcation on middle third, and 32.13% (n = 71) had bifurcation on cervical third. The examination of root canal systems of the teeth was based on Vertucci’s classification type of canal: 64.58% (#31) of the cases had type IV (2-2-2), 25.00% (#12) type II (2-2-1), 8.33% (#4) type I (1-1-1), and 2.09% (#1) type III (1-2-1). Conclusion: This study showed a high incidence of two-rooted maxillary first premolars collected from different regions in Kosovo. Internal root canal system morphology reflects the external root anatomy. Furthermore, there is correlation between the shape of the outer surface of the root and the shape of the root canal. The cases with one root of the maxillary first premolar with a deep depression on the mesial side contain a double root canal system more often than a single canal.
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