Experiment study of effect of Valeriana officinalis var. latifolia on expression of C-Fos,C-Jun in hippocampus zone after focal cerebral ischemia

摘要

AIM: To study influence of Valeriana officinalis var. latifolia(VOL) on expression of C Fos,C Jun after focal cerebral ischemia. METHODS: Inducing rat model of reversible middle cerebral artery occlusion(MCAO)using Koizumi’s intraluminal suture occlusion method.48 male rats were divided into 5 groups randomly, pseudo operation group, MCAO group, saline control group,VOL group.2 hours after MCAO, we took gastric gavage with VOL and saline, 8 hour per time, and took out of brain to test C Fos,C Jun expression immunohistochemically at the 5th day after operation.RESULTS: There was no positive cell in each hippocampus zone of normal group;we observed C Fos, C Jun positive cells in each Hippocampus zone after MCAO;Density of C Fos,C Jun positive cells of VOL group were apparently lower than that of simple ischemia group. CONCLUSION: VOL can relieve histopathological lesions after cerebral ischemia and promote protection function of rat through inhibiting the expression of C Fos,C Jun expression.