Investigation of the Role of ABC Transporters in Pyrethroids Resistance in the Major Malaria Vector Anopheles coluzzii from Northern Nigeria

摘要

Resistance to currently available insecticides in the major malaria vectors like Anopheles coluzzii is seriously reducing the effectiveness of core vector control tools. Overexpression of ATP binding cassette transporters (ABC transporters) has been implicated in insecticides resistance in the major malaria vector Anopheles funestus and An. gambiae. To identify the potential role of ABC transporters in pyrethroids resistance in this work, we use verapamil-a p-glycoprotein inhibitor in a synergist bioassay with pyrethroids on one An. coluzzii populations from northern Nigeria. Genomic DNA extraction and SINE 200 PCR established that the Anopheles s. l. from Auyo, Jigawa State was An. coluzzii. Pre-exposure to verapamil followed by Permethrin results in 0.12% knockdown after 1 hour exposure and 10.7% mortality after 24 hours, which was higher than that for permethrin alone, but not statistically significant. This bioassay result reveals that ABC transporters possibly do not contribute to the resistance.