Background: Suppression of physiologic myocardial sequestration of glucose, and hence the 2-deoxy-18 fluorodeoxyglucose (FDG) is of critical importance to effectively evaluate intrinsic cardiac pathology and better delineate extra- cardiac FDG activity on Positron Emission Tomography (PET) imaging. In a rodent model, we studied the effect of duration of fasting with or without high fat diet (HFD) consumption on myocardial FDG uptake. Methods: 9 Sprague- Dawley rats underwent four different preparation protocols before obtaining micro PET imaging: Non-fasting (NF), 18-hrs/Prolonged fasting (PF), 12-hrs/Short fasting followed by High Fat Diet (SF-HFD) and 18-hrs/Prolonged fasting followed by High Fat Diet (PF-HFD). Region of interest were drawn on the myocardium (heart) and ascending aorta (blood pool) to generate maximum standard uptake values (SUVm) for the heart (H-SUVm) and blood pool (BP- SUVm). Results: PF-HFD and SF-HFD preparation protocols resulted in significantly lower H-SUVm as compared to PF and NF protocols with H-SUVm of 1.49, 1.56, 4.38 and 10.19 respectively. Conclusion: PF-HFD and SF-HFD preparation protocols provide superior suppression of myocardial FDG uptake in comparison to PF and NF protocols. These findings offer an approach to study intrinsic cardiac disorders (vascular, infiltrative etc) and also provide better visualization of extra-cardiac pathologic disorders.
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