Polymorphism of rs1044925 in the acyl-CoA:cholesterol acyltransferase-1 gene and serum lipid levels in the Guangxi Bai Ku Yao and Han populations

摘要

Background The association of rs1044925 polymorphism in the acyl- CoA:cholesterol acyltransferase-1(ACAT-1) gene and serum lipid profiles is not well known in different ethnic groups.Bai Ku Yao is a special subgroup of the Yao minority in China.The present study was carried out to clarify the association of rs1044925 polymorphism in the ACAT-1 gene and several environmental factors with serum lipid levels in the Guangxi Bai Ku Yao and Han populations. Methods A total of 626 subjects of Bai Ku Yao and 624 participants of Han Chinese were randomly selected from our previous stratified randomized cluster samples.Genotyping of rs 1044925 polymorphism in the ACAT-1 gene was performed by polymerase chain reaction and restriction fragment length polymorphism combined with gel electrophoresis,and then confirmed by direct sequencing.Results The levels of serum total cholesterol(TC),high-density lipoprotein cholesterol (HDL-C),apolipoprotein(Apo) AI and ApoB were lower in Bai Ku Yao than in Han(P0.01 for all).The frequency of A and C alleles was 79.0%and 21.0%in Bai Ku Yao,and 87.3%and 12.7%in Han(P0.001);respectively.The frequency of AA,AC and CC genotypes was 63.2%,31.4% and 5.2%in Bai Ku Yao,and 75.6%,23.2%and 1.1%in Han(P0.001);respectively.The levels of TC,LDL-C and ApoB in Bai Ku Yao but not in Han were different between the AA and AC/CC genotypes in females but not in males (P0.05 for all).The C allele carriers had lower serum TC, LDL-C and ApoB levels as compared with the C allele non-carriers. The levels of TC,LDL-C and ApoB in Bai Ku Yao but not in Han were correlated with genotypes in females but not in males(P0.05 for all).Serum lipid parameters were also correlated with sex,age,body massindex,alcohol consumption, cigarette smoking,and blood pressure in both ethnic groups(P0.05-0.001).Conclusions These results suggest that the polymorphism of rs1044925 associated with female serum TC,LDL-C and ApoB levels in the Bai Ku Yao population.The C allele carriers had more favorable serum TC,LDL-C and ApoB levels than the C allele noncarriers. in the ACAT-1 gene is