Bacteriological Analysis of Well Water Sources in the Bambui Student Residential Area

摘要

Majority of the population in the Bambui student residential area depend on wells as their source of water supply. Due to increasing complaints from students after using this well water sources, this study was carried out to examine the microbial quality of well water sources in the Bambui student residential area, Bamenda, Cameroon as a way of safe guarding public health against water borne diseases. Water samples from a total of fifteen wells, covered and uncovered were assessed for their bacteriological quality using the multiple tube fermentation technique to determine the coliform count using the most probable number method. Positive tubes of the presumptive test were further cultured on appropriate solid media. The organisms isolated were further characterized using standard procedures. The result of the study revealed that most of the wells were grossly contaminated with bacteria pathogens such as Klebsiella species (95%), Escherichia coli (52%) and Salmonella typhi (32.5%). Comparatively, the uncovered wells were more contaminated with bacteria pathogens than the covered well. All the water samples except three exceeded the standard limit of the most probable number (MPN) per 100 ml set for untreated drinking water by the World Health Organization. This result highlight the fact that most well water sources in the Bambui student residential area metropolis are not safe microbiologically for drinking without additional treatment such as boiling or disinfection and this could lead to outbreak of water borne diseases. Good and proper environmental and personal hygiene must be maintained especially by the users of these wells to prevent their contamination with bacterial pathogens. Wells should also be properly constructed.