Cytogenotoxic Effect of Pesticides Induces Variability in Micronu­cleus and Nucleo-Cytoplasmic Abnormalities in Channa punctatus in vivo

摘要

Our aim was to study the genotoxic,cytotoxic and clastogenic effect of locally used various groups of agro-pesticides in the protection of crops using fresh water fish Channa punctatus as test model to estimate water pollution by micronucleus(MN)assay in vivo.Three different concentrations(MC,MC/2&MC/5)of eight pesticides(Dimethoate,Dichlorovos,chlorpyriphos and Malathion,Methyl parathion,Fenvalerate,Cypermethrin and Carbaryl)at different time periods(5,10,15,20,25 days)were treated.Peripheral blood samples smears of fish were collected and stained with Giemsa,micronuclei and nucleo-cytoplasmic abnormalities were analyzed under Germany made Leitz microscope.Qualitative analysis shows the rate of concentration,period,nature and mode of action of different agro-pesticides induces varieties of micronuclei and nucleo-cytoplasmic abnormalities even in the same species of fish Channa punctatus in the context of same/different pesticides in different species.