Analysis of Two-Dimensional Gel Electrophoresis Map of Methicillin-Resistant Staphylococcus aureusTreated with Acetone Extract from Canarium odontophyllumMiq. Leaves

摘要

Methicillin-resistant Staphylococcus aureus (MRSA) infection is a global health concern that has caused severe health threats over the past decade. Leaves extract of C. odontophyllum has been proven previously as an anti MRSA agent. Proteomics provide a technique that used to analyze the differential of protein expression profile between untreated and treated MRSA with subinhibitory concentrations of acetone extract from C. odontophyllum leaves. This study aims to determine the optimum parameter for analysis of protein expression profile using two-dimension gels electrophoresis (2-DE) for MRSA protein after treatment with acetone extract from style="font-family:Verdana;">C style="font-family:Verdana;">. style="font-family:Verdana;"> odontophyllu style="font-family:Verdana;">m style="font-family:Verdana;">leaves. Comparison of the Protein Expression Profile (PEP) between the untreated and treated MRSA was analyzed using PDQuest software. The optimum condition for MRSA protein treated with acetone extract from style="font-family:Verdana;">C style="font-family:Verdana;">. style="font-family:Verdana;"> odontophyllum style="font-family:Verdana;">leaves to produce the best resolution with greater spot distribution was as follows: 100 μg volume of MRSA protein that loaded after passive rehydration then was run until reaching 25 kVrhs during IEF using 17 cm IPG strip within ranges of pH 4 - 7. Analysis of protein expression from the 2-DE gel map shows that 9 protein spots up-regulated and 41 protein spots were down-regulated with more than 2-fold differences (p < 0.05). This preliminary study on the PEP of MRSA treated with acetone extract of style="font-family:Verdana;">C style="font-family:Verdana;">. style="font-family:Verdana;"> odontophyllum style="font-family:Verdana;">leave may provide an insight into the antimicrobial mechanism, which could lead to the identification of target protein for future novel therapeutic development against MRSA infections.