Morphofunctional Properties of Human Platelets, Treated with Low Doses of Hydrogen Peroxide

摘要

We studied morphofunctional properties of human platelets, treated with low doses of hydrogen peroxide (H2O2). It was shown that platelet incubation with 20 - 75 μM H2O2 during 24 hours at 20°C - 22°C did not cause visible structural deficiencies in cells. Platelets maintained their adhesion activity and velocity of degranulation at 20 μM H2O2. 30 - 60 μM H2O2 dose-dependently reduced lamella’s growth and allowed retaining granule bulk inside cytoplasm of spreading platelets. After 1 - 2 hours, the most effect of platelet granules’ stabilization was registered at samples, previously incubated with 60 μM H2O2. Adrenalin activation led to rapid degranulation of stabilized platelets, whereas lamella’s growth in activated platelets was low or medium.