Spatially resolved and multiplexed MicroRNA quantification from tissue using nanoliter well arrays

摘要

Spatially resolved gene expression patterns are emerging as a key component of medical studies,including companion diagnostics,but technologies for quantification and multiplexing are limited.We present a method to perform spatially resolved and multiplexed microRNA (miRNA) measurements from formalin-fixed,paraffin-embedded (FFPE) tissue.Using nanoliter well arrays to pixelate the tissue section and photopatterned hydrogels to quantify miRNA,we identified differentially expressed miRNAs in tumors from a genetically engineered mouse model for nonsmall cell lung cancer (K-rasLSL-G12D/+;p53fl/fl).This technology could be used to quantify heterogeneities in tissue samples and lead to informed,biomarker-based diagnostics.