Electrospun CNT embedded ZnO nanofiber based biosensor for electrochemical detection of Atrazine:a step closure to single molecule detection

摘要

In this study we have reported the design and development of a facile,sensitive,selective,and label-free electrochemical sensing platform for the detection of atrazine based on MWCNT-embedded ZnO nanofibers.Electrospun nanofibers were characterized using scanning electron microscope (SEM),transmission electron microscope (TEM),X-ray diffraction (XRD),X-ray photoelectron spectroscope (XPS),UV-Visible spectroscope (UV-VIS),and Fourier-transform infrared spectroscope (FTIR).Electrochemical properties of MWCNT-ZnO nanofiber-modified electrodes were assessed using electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV).Binding event of atrazine to anti-atrazine antibody,which immobilized on nanofiber-modified electrode via EDC and NHS chemistry,was transduced with EIS.Due to high conductivity,surface area,and low bandgap of MWCNT-ZnO nanofibers,we have achieved the sensitivity and limit of detection (LoD) of sensor as 21.61 (KΩ μg-1 mL-1) cm-2 and 5.368zM for a wide detection range of 10zM-1 μM.The proposed immunosensing platform has good stability,selectivity,repeatability,and reproducibility,and are less prone to interference.