Long Non-coding RNA HOTAIR for Tumor Diagnosis and Pathological Staging

摘要

LncRNA HOTAIR has different expression levels in different stages of tumorigenesis and development.Therefore,it has potential application in clinical diagnosing tumor stage as a tumor marker.Fifty patients with lung cancer and fifty healthy volunteers were selected as lung cancer group and control group respectively.According to T and N stage of cancer,the patients were divided into T1,T2,T3 and N0,N1,N2 groups.Fluorescence in situ hybridization(FISH)was used to detect the expression of HOTAIR in lung cancer tissues and paraneoplastic tissues.The expression levels of NSE,CEA and CYFRA21-1 in serum were detected by immunofluorescence.The results showed that the expression level of HOTAIR in paraneoplastic tissues(0.98±0.04)were significantly lower than that in lung cancer tissues(3.56±0.15).The serum levels of NSE,CEA and CYFRA21-1 of the lung cancer group were higher than those in the control group.The concentrations of HOTAIR,NSE,CEA and CYFRA21-1 increased with the progress of clinical stages,which indicated that the expression of HOTAIR was positively correlated with the expression of SE,CEA and CYFRA21-1.These results indicate that HOTAIR,NSE,CEA and CYFRA21-1 are associated with the initiation and development of tumors.Therefore,HOTAIR combined with tumor marker can improve the accuracy of detection of pathological stage of lung cancer.